Israel is house to only seven species of amphibians: the green toad Bufo viridis, the treefrog Hyla savignyi (H. arborea), the marsh from Pelophylax bedriagae(Pelophylax ridibundus Rana levantina), the eastern spadefoot Toad Pelobates syriacus, the southern banded newt Ommatotriton vittatus (Triturusvittatus), the Fire Salamander Salamandra salamandra as well as the recently extinct Israel painted frog, Discoglossus nigriventer

 The amphibian collections are likewise the smallest of the tetrapod collections at Tel Aviv Zoological Museum, but still holds 2,300 specimens. Approximately 75% of the specimens originate from the Israel, and the collection has ample samples (>100 specimens, > 200 for 4 species) of all extant Israeli species (the only specimens of the extinct Discoglossus nigriventer in existence are housed at the natural history collections of the Hebrew University in Jerusalem). As Israel is the at the southern distribution limit for some of these species these population represent interesting evolutionary units. Other large collections are from Turkey and Italy, and altogether the collection houses about 100 species collected over the last 75 years.

Many of the amphibians were collected by the late professor Mendelssohn, one of the founders of Tel Aviv University, and the pioneers of nature conservation in Israel, who also founded the natural history collections in Tel Aviv University.